Assessment of Bacteriophage-induced Inflammatory Mediators in Salmonella -infected Chicken Macrophage HD11 Cells

摘要

This study was designed to characterize the role of P22 in the intracellular survival and pro-inflammatory responses in Salmonella -infected chicken macrophage-like HD11 cells. The intracellular survival of Salmonella Typhimurium was evaluated in the presence or absence of P22 at 24 h postinfection (hpi). The production of inflammatory mediators, including nitric oxide (NO), IL-1 β , IL-6, IL-8, and IL-10, was determined at 0 and 24 hpi by using ELISA kits. The survival rates of intracellular S . Typhimurium were effectively reduced to 8.1% in Salmonella -infected HD11 cells treated with P22 at 24 hpi. The secretion of NO, IL-6, IL-8, and IL-10 varied with infection period and bacteriophage treatment. The reduction of intracellular S . Typhimurium was highly correlated with the enhanced production of NO ( r ＝0.816, P <0.001). The results suggest that bacteriophage can be used to control the intracellular pathogens. This study would provide useful information on the bacteriophage-host interactions and open the door for designing an effective and safe bacteriophage therapeutic system.