Multimodal combinational holographic and fluorescence fluctuation microscopy to obtain spatial super-resolution

摘要

Waysofcombinationofholographicandsuper-resolutionfluorescenttechniquesinthesameopticalschemearedescribed.Thekeyparametersinfluencingachievementofmaximumpossibleresolutionareconsidered.Thepossibilitytochoosedifferentfluorescencetechnicfordifferenttypesoffluorophoreswithoutanyopticalschemechangesispresented.Asaresultincaseofvisualizationofthesamples,whichtransparentinopticalband,three-dimensionalsuperresolutionisreceivedthatsignificantlyexpandspossibilitiesofthenoninvasiveanalysisofbiologicalsamples...