Extended kinetic model of real-time polymerase chain reaction process

摘要

Real-timepolymerasechainreaction(real-timePCR)isthemainmoleculargeneticmethodusedforqualitativeandquantitativeanalysisofspecificnucleicacidsequencesinmanyareasofbiomedicalresearch.TheoreticalstudyofpCrmodelsallowstoestimatetheinfluenceofvariousreactioncomponentsandparameters,andtodeterminetheunknownparametervaluesbyapproximatingtheexperimentalreal-timePCRcurves.Anextendedkineticmodelofreal-timePCRispresented.ThemodeltakesintoaccounttheenzymeactivitybasedonMichaelis-Mentenkinetics,thehybridizationofcomplementaryDNAfragments,thepresenceofafluorescentprobeusedfordetectionofthereactionproducts,andthetemperaturedependenceofprimersandprobehybridization...