Differentiation of banding patterns between Streptococcus mutans and Streptococcus sobrinus isolates in rep-PCR using ERIC primer

摘要

Background:StreptococcusmutansandStreptococcussobrinusareconsideredtobeimportantbacterialspeciesintheinitiationofhumandentalcaries.Therefore,theestablishmentofareliablegenotypingmethodtodistinguishS.mutansfromS.sobrinusisofcentralimportance.Objective:Weassessedtheusefulnessofrepetitiveextragenicpalindromicpolymerasechainreaction(rep-PCR)usingERICprimerbandingpatternsindifferentiatingS.mutansandS.sobrinus.Design:FiveS.mutansandtwoS.sobrinusprototypestrainsand50clinicalisolates(38S.mutansserotypec,4S.sobrinusserotyped,and8S.sobrinusserotypeg)wereexamined.Thebandingpatternsofampliconsgeneratedwerecomparedamongtheprototypestrainsandclinicalisolates,tofindcommonbandsthatdistinguishS.mutansandS.sobrinus.Results:Multiplebandingpatternswereseenwithallstrainstested.TherepresentativestrainsofS.mutanstestedrevealedsixunique,strongbandsat2,000bp,1,700bp,1,400bp,1,100bp,850bp,and250bp,whereasS.sobrinushadsevenstrongbandsat2,000bp,1,800bp,1,100bp,900bp,800bp,600bp,and550bp.Thebandat1,100bpwastheonlybandthatwasobservedinbothS.mutansandS.sobrinus.Furthermore,mostclinicalS.mutansisolatesrevealedidenticalbandingpatterns.AllS.mutanshadampliconsat1,700bp,850bp,and250bp,whereasthoseofS.sobrinuswereat1,100bp,900bp,and800bp.Conclusions:Theseresultsindicatethatusingrep-PCRwiththeERICprimerscandistinguishbetweenS.mutansandS.sobrinus.Keywords:rep-PCR;mutansstreptococci;clinicalisolates;dentalcaries;amplicons(Published:2December2011)Citation:JournalofOralMicrobiology2011,3:7190-DOI:10.3402/jom.v3i0.7190