Dense Lamellar Scaffold as Biomimetic Materials for Reverse Engineering of Myocardial Tissue: Preparation, Characterization and Physiomechanical Properties

摘要

The aim of this study was to develop dense lamellar scaffold as a biomimetic material for myocardial tissue regeneration. To generate dense lamellar and porous structure with biomimetic polymers was used plastic compression method. The polymeric blends of collagen, chitosan and silk fibroin were used to obtain three-dimensional devices with interconnected porous, anisotropy, and anatomical similarity with the extracellular matrix. The texture profile analysis was employed to investigate the physiomechanical properties, including mucoadhesion and swelling efficiency. Fourier transform infrared spectroscopy, differential scanning calorimetry, computerized microtomography, scanning electron microscopy were employed to investigate the structural properties, surface morphology, porosity of the dense lamellar scaffold and, cell viability and image cytometer with H9c2 cells (cardiac myoblasts). The swelling efficiency of blends was evaluated using Enslin dispositive. The physiomechanical properties associated with swelling efficiency, porosity, anisotropy degree and, cell proliferation and viability (in vitro) suggest that the scaffold with COL-CH-SF obtained by plastic compression may be a potential biomimetic material for reverse engineering of myocardial tissue.