The aim of this work was optimization of lime pretreatment parameters such as temperature, time and reaction ratio to maximization of reducing saccharide yields occurred by enzyme hydrolysis of pretreated plant material (wheat straw). Pretreatment conditions were optimized using response surface methodology. The optimal conditions were chosen to promote reducing saccharide yields following enzymatic digestion and they were temperature 91.5 °C, time 2.4 hours and reaction ratio 19.7 mL to 1 g of treated wheat straw. The experimental values agreed with predicted within a 95 % confidence interval. The computed model of wheat straw pretreatment by lime can be used for the effective utilization of secondary products obtained in agriculture sector.
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