In vitro thrombolytic activity, antioxidant and cytotoxic properties of fruit extracts of Ficus erecta (Thunb.)

摘要

The aim of the study was to evaluate different biological properties of fruits extracts of Ficus erecta (Thunb.). The concentrated methanolic extracts and Kupchan partitions were screened for thrombolytic activity, whereas streptokinase was used as standard antioxidant property by evaluating free radical scavenging effect and determining total phenolic content and cytotoxic activity by brine shrimp lethality bioassay. The highest thrombolytic activity was exhibited by carbon tetrachloride soluble partition (CTP) of the plant (27.53±0.41%) against the standard streptokinase (65.16±0.064%). The IC50 value of the petroleum ether soluble partition (PEP) was found to be 7.35±0.08 μg/ml, whereas standard ascorbic acid demonstrated IC50 value of 5.80±0.22 μg/ml. The petroleum ether soluble partition (PEP) also exhibited highest phenolic content (43.69±1.4 mg of GAE/100 g of extractives). A correlation was calculated between total phenolic content and free radical scavenging activity of F. erecta which showed positive result having correlation coefficient (R2) of 0.779. Among the LC50 values of the plant material, petroleum ether soluble partition (PEP) exhibited the lowest value of 1.20±0.028 μg/ml as compared to the standard vincristine sulphate (0.45?±0.003 μg/ml). The present study showed that, the plant F. erecta demonstrated strong cytotoxic and antioxidant property and moderate thrombolytic activity.