Anti fungal activity of alcoholic extract of Peganum harmala seeds

摘要

In our study, it was aimed to determine antifungal activity of?Peganum?harmala?on medically important yeasts and molds?in vitro.?Fungal suspensions of?Candida?spp. and aspergillus spp. isolated from the clinical specimens were treated with serial dilutions of?P. harmalaalcoholic extract in Sabouraud Broth. The titers included: 1/20, 1/40, 1/80, 1/160, 1/320, 1/640, and incubated for 24 h at 30°C. Anti fungal activity of the extract was determined as MIC and MFC. For the determination of MFC, a small volume of above serial dilutions was cultured on SGA 4% medium. After 24 to 72 h of incubation at 30°C, the cultures were looked for fungal growth. The highest and lowest inhibitory effects of?P. harmala?extract?were determined on?C. glabrata?(MIC: 0.312 mg/ml) and?C. albicans?(MIC:?1.25 mg/ml), respectively. The MFC for?Candida?species was differed from 0.62 to 2.5 mg/ml. The highest fungicidal effect was seen on?C. glabrata?and?C. tropicalis?(MFC: 0.62 and 0.125 mg/ml, respectively) and the lowest was for?C. albicans?(MFC: 2.5 mg/ml). The treatment of Aspergillus suspensions with Perganum extract showed a decreasing of growth and sporolation without definite inhibition. The alcoholic extract of?P. harmala?showed a fungicidal activity on opportunistic yeasts,?Candida?spp., and a decreasing sporolation for aspergillus most important species.