Molecular Characterization of Seven Different Species of Aspergillus through Random Amplified Polymorphic DNA (RAPD) and Enzyme Analysis

摘要

The aim of this study was to characterize seven species of Aspergillus at molecular level, using random amplification of polymorphic DNA (RAPD). RAPD- PCR conditions were optimized for two primers of series B, GL Decamer B-09 and GL Decamer B-10 out of 10 total primers. RAPD results were evaluated by a statistical software Minitab and a phylogenetic tree was prepared. GL Decamer B-09 showed 38 bands and GL Decamer B-10 gave 46 bands, showing 50% and 57% similarity respectively, among species. Biochemical characterization was done by screening of zones production with particular enzyme activity of each specie resulted in particular substrate degradation.