Atrazine Induced Morphological Alterations in Spermatocytes of Goat in vitro

摘要

The presence of pesticide residues in food, in soils and sediments as well as in run-off water are ubiquitous environmental toxicants that pose a risk to human health. Aim of the present investigation was to study the effect of different doses of atrazine on spermatocytes of Capra hircus in vitro . Small pieces (approximately 1 mm³) of testicular tissue were divided into one experimental and one control group; the experimental group was treated with atrazine (1x10^-3, 1.0 and 100 nmol mL^-1) and exposed for different durations. Pycnosis, chromolysis and vacuoles of varied sizes and shapes were frequently observed in spermatocytes due to the atrazine exposure. All the degenerating changes increased as the exposure duration was increased from 1 to 4 and 4 to 8 h. The relative frequency of atretic spermatocytes was 30, 38 and 44% after 1, 4 and 8 h, respectively at 1x10^-3 nmol mL^-1 atrazine concentration. Ultra-structurally, shrinkage in cytoplasm of zygotene primary spermatocytes was distinct and prominent, chromolysis, ruptured membrane of turgid mitochondria and Golgi bodies were scattered in the spermatocyte cytoplasm.