Allele Frequency and Molecular Genotypes of ABO Blood Group System in a Jordanian Population

摘要

To determine the phenotypic, allelic frequencies and the genotypes of ABO blood groups in a Jordanian population, samples of 12215 randomly healthy Jordanian voluntary blood donors during the period 1998-2003 were taken from the National Blood Bank donor registry, Amman, Jordan. The results of the phenotypic distribution indicated that 4686 (38.36%) of the donors were type A, 4473 (36.62%) O, 2203 (18.04%) B and 853 (6.98%) AB. The gene frequencies were 0.6052 for I^o allele, 0.2607 for I ^a allele and 0.1341 for I^b allele. Using PCR-RFLP technique, two separate segments of the transferase gene containing nucleotide 261 in exon 6 and nucleotide 703 in exon 7 of the ABO gene locus were amplified and their products were analyzed with two restriction enzymes ( Kpn I and Alu I). The electrophoresis patterns of 105 samples showed that ABO genotypes were AA : 6 (5.714%), AO : 35 (33.333%), BB : 1 (0.953%), BO : 14 (13.333%), AB : 10 (9.524%) and OO : 39 (37.143%).