The Effect of Brotowali Stem Extract (Tinospora Crispa) Towards Increasing Number of Lymphocytes in the Healing Process of Traumatic Ulcer on Diabetic Wistar Rat

摘要

One of the common complication of diabetesis delayed wound healing in oral traumatic ulcer.Lymphocyte cell plays an important role in late inflammatory phase during the process of woundhealing. Brotowali (Tinospora crispa) contrains flavonoid and terpenoid which can help to controlblood glucose levels and accelerate wound healing. Tinospora crispa extract was made with oven-dried method at 50°C. Dry specimens are then made into a powder and macerated with ethanol80% (1:10 w/v) The experimental animals were divided into 3 groups, control group 1: NormalWistar Rat with traumatic ulcer, control group 2:DiabeticWistar rat with traumatic ulcer, andtreatment group: Diabetic Wistar rat with traumatic ulcer treated with 250 mg/kg Tinospora crispaextract once a day. All sample were euthanized on day 3, 5, and, 7 after traumatic ulcer was made.Then histopathology preparation was made to count the number of lymphocytes. Blood glucoselevels measurement was conducted on day 3, 5, 7, and 14 after traumatic ulcer was made. Kruskal-Wallis test showed a significant difference of blood glucose levels between the control group 1,control group 2 and treatment group. One Way Anova test showed a significant difference ofnumber of lymphocytes between control group 1, control group 2 and treatment group. Brotowalistem extract (Tinospora crispa) is proven to affect blood glucose levels on day 3, 5, 7, and 14. It isalso proven that Brotowali stem extract affect the number of lymphocytes in healing of traumaticulcer among diabetic Wistar rats on day 3, 5, and 7.