Clinical value of ELISA detection of serum Exosome and Dickkopf-1 content in patients with gastric cancer

摘要

Objective: To study the correlation of serum Exosome and Dickkopf-1 content withpathological process and gene expression in patients with gastric cancer. Methods: A totalof 480 patients diagnosed with gastric cancer in our hospital between May 2015 and July2016 were selected as the gastric cancer group and 220 healthy volunteers receiving physicalexamination during the same period were selected as control group. Serum was collected todetect Exosome and Dickkopf-1 content, and gastric cancer tissue and para-carcinoma tissuewere collected to detect the expression of pro-proliferation and pro-invasion molecules as wellas tumor suppressor genes. Results: Serum Exosome and Dickkopf-1 content of gastric cancergroup were significantly higher than those of control group and the deeper the infiltrationand the worse the differentiation, the higher the serum Exosome and Dickkopf-1 content,and serum Exosome and Dickkopf-1 content in patients with lymph node metastasis weresignificantly higher than those in patients without lymph node metastasis; UHRF1, SIRT1and LOXL2 expression in gastric cancer tissue were significantly higher than those in paracarcinomatissue and positively correlated with serum Exosome and Dickkopf-1 content whileRASAL2, ING5, Period1 and Period2 expression were significantly lower than those in paracarcinomatissue and negatively correlated with serum Exosome and Dickkopf-1 content.Conclusion: The elevated serum Exosome and Dickkopf-1 in patients with gastric cancer areclosely related to the high expression of pro-proliferation and pro-invasion molecules as wellas the low expression of proto-oncogene.