Correlation between peripheral blood Cx40 gene polymorphism and atherosclerotic plaque property development in patients with cerebral infarction

摘要

Objective: To study the correlation between peripheral blood connexin 40 (Cx40) gene polymorphism and atherosclerotic plaque property development in patients with cerebral infarction. Methods: Patients who were treated in the Second Affiliated Hospital of Xi'an Medical University due to acute cerebral infarction between March 2015 and March 2018 were selected as cerebral infarction group, and healthy subjects who received physical examination during the same period were selected as control group. Peripheral blood was collected to detect the polymorphism of Cx40 gene rs35594137 locus, and serum was collected to determine the contents of cytokines, proteases and related molecules. Results: The constituent ratio of Cx40 gene AA+AG genotype in peripheral blood of cerebral infarction group was higher than that of control group whereas the constituent ratio of GG genotype was lower than that of control group; serum IL-17, HMGB1, VCAM1, MCP-1, P-selectin, YKL-40, MMP9, TIMP1 and Caspase-3 contents as well as MMP9/TIMP1 ratio of cerebral infarction group were significantly higher than those of control group whereas ADAMTS13 and Vaspin contents were significantly lower than those of control group; serum IL-17, HMGB1, VCAM1, MCP-1, P-selectin, YKL-40, MMP9, TIMP1 and Caspase-3 contents as well as MMP9/TIMP1 ratio of cerebral infarction group of patients with CX40 gene AA+AG genotype were significantly higher than those of patients with GG genotype whereas ADAMTS13 and Vaspin contents were significantly lower than those of patients with GG genotype. Conclusion: The mutation from Cx40 gene rs35594137 allele G to A in peripheral blood of patients with cerebral infarction can promote the development of atherosclerotic plaque properties.