The apoptosis and autophagy in rats with spinal cord injury and the intervention effect of ferulic acid

摘要

Objective: Too assess the apoptosis and autophagy in rats with spinal cord injury and studythe intervention effect of ferulic acid. Methods: Adult male SD rats were selected and dividedinto sham operation group (Sham group), spinal cord injury group (SCI group) and ferulicacid intervention group (FA group), the models of spinal cord injury were made by bulldogclamp clamping and given intragastric administration of 100 mg/kg ferulic acid twice a dayfor intervention. 7 d and 14 d after intervention, the expression of apoptosis molecules, antiapoptosismolecules and autophagy molecules in spinal cord were determined. Results:PARP-1, AIF, ASK-1, mTOR, PI3K, Akt, Beclin1 and LC3-II protein expression in spinal cordtissue of SCI group were significantly higher than those of Sham group while Survivin, NAIPand Bcl-2 protein expression were significantly lower than those of Sham group; PARP-1,AIF, ASK-1, mTOR, PI3K, Akt, Beclin1 and LC3-II protein expression in spinal cord tissueof FA group were significantly lower than those of SCI group while Survivin, NAIP and Bcl-2protein expression were significantly higher than those of SCI group. Conclusion: Ferulic acidcan inhibit cell apoptosis and autophagy in spinal cord injury.