Evaluation value of intestinal flora detection for intestinal mucosal inflammation and immune response in patients with ulcerative colitis

摘要

Objective: To study the evaluation value of intestinal flora detection for intestinal mucosalinflammatory response and immune response in patients with ulcerative colitis. Methods: Thepatients who were diagnosed with ulcerative colitis in Zigong Fifth People’s Hospital betweenMarch 2015 and February 2017 were selected as the UC group, and those who were diagnosedwith colonic polyps were selected as the control group. Fresh excreta were collected to detectthe number of intestinal flora, and the diseased intestinal mucosa tissue was collected to detectthe expression of inflammatory response molecules and immune cell transcription factors.Results: enterococcus contents in intestinal tract and TLR4, NF-kB, TNF-α, HMGB-1, T-betand RORC mRNA expression levels in intestinal mucosa of UC group were significantlyhigher than those of control group while bifidobacteria contents in intestinal tract and SOCS2,SOCS3, Foxp3 and GATA-3 mRNA expression levels were significantly lower than thoseof control group; TLR4, NF-kB, TNF-α, HMGB-1, T-bet and RORC mRNA expressionlevels in intestinal mucosa of UC patients with grade II and grade III flora disturbance weresignificantly higher than those of UC patients with normal flora and grade I flora disturbancewhile SOCS2, SOCS3, Foxp3 and GATA-3 mRNA expression levels were significantly lowerthan those of UC patients with normal flora and grade I flora disturbance; TLR4, NF-kB,TNF-α, HMGB-1, T-bet and RORC mRNA expression levels in intestinal mucosa of UCpatients with grade III flora disturbance were significantly higher than those of UC patientswith grade II flora disturbance while SOCS2, SOCS3, Foxp3 and GATA-3 mRNA expressionlevels were significantly lower than those of UC patients with grade II flora disturbance.Conclusion: The intestinal flora disturbance in patients with ulcerative colitis can result ininflammatory response activation and immune response disorder.