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Performance of Quantitative Buffy Coat, QBC Fluorescence and Staining Technologies? Test, and SD Bioline? Malaria Rapid Test in Malaria Diagnosis in Western Kenya

机译:Buffy定量外套的性能,QBC荧光和染色技术?测试和SD Bioline?肯尼亚西部疟疾诊断中的疟疾快速检测

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Malaria remains the most important parasitic disease in sub-Saharan Africa as a cause of morbidity and mortality. Effective management of malaria relies on prompt and accurate diagnosis to guide treatment. The World Health Organization (WHO) recommends that all suspected malaria cases be tested before initiation of treatment, thus diagnosis of malaria requires parasitological confirmation of malaria parasites in the blood of suspected patients. This cross-sectional study conducted at the Ahero County Hospital, Kisumu, Kenya, evaluated the performance of quantitative buffy coat (QBC) (QBC Fluorescence and Staining Technologies?(QBC F.A.S.T.?)-improved QBC system and SD Bioline? malaria rapid tests) against 'gold standard' (Giemsa blood stained slides microscopy) for the detection of Plasmodium species in children
机译:疟疾仍然是撒哈拉以南非洲最重要的寄生虫病,是发病和死亡的原因。疟疾的有效管理依靠及时准确的诊断来指导治疗。世界卫生组织(世卫组织)建议在开始治疗之前对所有可疑疟疾病例进行检查,因此,要对疟疾进行诊断,需要寄生虫学确认可疑患者血液中的疟原虫。此横断面研究在肯尼亚基苏木的Ahero县医院进行,评估了定量血沉棕黄层(QBC)(QBC荧光和染色技术?(QBC FAST?)改进的QBC系统和SD Bioline?疟疾快速检测)的性能。反对“金标准”(吉姆萨血染玻片显微镜)检测肯尼亚西部疟疾流行地区五岁以下儿童(n = 385)的疟原虫种类。在整个测试和金标准(显微镜)中,对差异较大的样品进行了实时PCR.QBC,QBC F.A.S.T.?和SD Bioline?疟疾快速检测分别为90%(95%CI:85-94),77%(95%CI:71-83)和91%(95%CI:86-94),而特异性为30%(95%) CI:24-37),83%(95%CI:77-88)和67%(95%CI:60-73)。阳性预测值(PPV)分别为58%(95%CI:52-63),83%(95%CI:77-88)和74%(95%CI:63-80)。尽管标准的QBC疟疾测试和(QV)疟疾测试分别为74%(95%CI:63-84),78%(95%CI:71-83)和87%(95%CI:81-92)。 SD Bioline?相对于改良后的QBC F.A.S.T。?,疟疾快速诊断测试(RDT)显示出更好的敏感性。测试中,后者具有更好的特异性。与显微镜相比,这些测试的性能仍然适中。

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