Effect of doxycycline intervention on the apoptosis as well as the IL-1, TNF-α and HIF-1α expression in cornea and aqueous humor in rats with corneal alkali burn

摘要

Objective: To study the effect of doxycycline intervention on the apoptosis as well as the IL-1,TNF-α and HIF-1α expression in cornea and aqueous humor in rats with corneal alkaliburn. Methods: Male SD rats were selected as experimental animals and randomly dividedinto control group, alkali burn group and doxycycline group, corneal alkali burn models wereestablished by sodium hydroxide eye drop and then they received doxycycline eye dropsintervention. The expression of apoptosis molecules, inflammatory response cytokines andangiogenesis moleculesin the cornea as well as the expression of inflammatory responsecytokines and angiogenesis molecules in aqueous humor were detected 14 and 28 d aftermodel establishment. Results: 14 and 28 d after model establishment, Bcl-2 and PEDF proteinexpression in cornea tissue of alkali burn group were significantly lower than those of controlgroup while Caspase-3, Caspase-8, Caspase-9, IL-1, TNF-α, HIF-1α and VEGF proteinexpression were significantly higher than those of control group; Bcl-2 and PEDF proteinexpression in cornea tissue of doxycycline group were significantly higher than those of alkaliburn group while Caspase-3, Caspase-8, Caspase-9, IL-1, TNF-α, HIF-1α and VEGF proteinexpression were significantly lower than those of alkali burn group. Conclusion: Doxycyclinefor corneal alkali burn intervention can inhibit the apoptosis, inflammatory response andangiogenesis.