Effect of doxycycline intervention on the apoptosis as well as the IL-1, TNF-α and HIF-1α expression in cornea and aqueous humor in rats with corneal alkali burn

摘要

Objective:To study the effect of doxycycline intervention on the apoptosis as well as the IL-1, TNF-α and HIF-1α expression in cornea and aqueous humor in rats with corneal alkali burn.Methods: Male SD rats were selected as experimental animals and randomly divided into control group, alkali burn group and doxycycline group, corneal alkali burn models were established by sodium hydroxide eye drop and then they received doxycycline eye drops intervention. The expression of apoptosis molecules, inflammatory response cytokines and angiogenesis moleculesin the cornea as well as the expression of inflammatory response cytokines and angiogenesis molecules in aqueous humor were detected 14 and 28 d after model establishment.Results: 14 and 28 d after model establishment, Bcl-2 and PEDF protein expression in cornea tissue of alkali burn group were significantly lower than those of control group while Caspase-3, Caspase-8, Caspase-9, IL-1, TNF-α, HIF-1α and VEGF protein expression were significantly higher than those of control group; Bcl-2 and PEDF protein expression in cornea tissue of doxycycline group were significantly higher than those of alkali burn group while Caspase-3, Caspase-8, Caspase-9, IL-1, TNF-α, HIF-1α and VEGF protein expression were significantly lower than those of alkali burn group.Conclusion: Doxycycline for corneal alkali burn intervention can inhibit the apoptosis, inflammatory response and angiogenesis.