The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat’s Spermatozoa after Freezing at -80°C

摘要

The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P_(0)), 10% (P_(1)), 20% (P_(2)), and 30% (P_(3)). The result showed that the percentage of sperms abnormality in P_(0), P_(1), P_(2), and P_(3) were respectively 8.67%, 6.67%, 5.67%, and 10%. P_(2) was the lowest percentage, while P_(3) was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours.Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.