首页> 外文期刊>Journal of Ginseng Research >Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by γ-irradiation (60Co) of Adventitious Roots
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Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by γ-irradiation (60Co) of Adventitious Roots

机译:不定根γ射线(60Co)诱导的野生人参突变体品系的人参皂甙生成及形态特征

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With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng ( Panax ginseng Meyer), the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg1, Re, Rb1, Rb2, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4 from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg1, Re, Rb1, Rb2, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture. Keywords: Panax ginseng , Adventitious root, γ-irradiation, Mutant, Ginsenosides.
机译:为了提高高丽参野山参不定根培养物中人参皂苷的含量,对根部进行了不同剂量的γ射线处理(5、10、25、50、75、100和200 Gy)。不定根的生长在100 Gy以上受到抑制。辐照的不定根在50至100 Gy时,形态参数和粗皂苷含量均出现显着变化。因此,基于表型形态和相对于野生型对照的粗皂苷含量,从用50Gy和100Gy处理的35个细胞系的繁殖中选择了四个突变细胞系。确定了7种主要人参皂苷的含量(Rg 1 ,Re,Rb 1 ,Rb 2 ,Rc,Rf和Rd)第1和3行来自100 Gy,第2和4行来自50 Gy。与烧瓶和生物反应器中的根系对照相比,细胞系2显示出更多的次生根,更长的长度和更高的生长速率。细胞系1显示出更大的平均直径,并且在生物反应器中的生长速率与对照相当,但是在烧瓶培养的根中更高。细胞株1和2,特别是前者,在瓶和生物反应器中显示出比对照更多的人参皂苷含量。因此,我们选择细胞系1来进一步研究人参皂甙的含量。与对照相比,烧瓶和生物反应器培养物中品系1的粗皂苷含量分别增加了1.4倍和1.8倍。 7种人参皂苷类型(Rg 1 ,Re,Rb 1 ,Rb 2 ,Rc,Rf和Rd)的总含量增加了1.8和分别为对照的2.3倍。与烧瓶培养物相比,生物反应器培养物中的粗皂苷和人参皂甙含量增加了约1.4倍。关键词:人参,不定根,γ射线辐射,突变体,人参皂甙。

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