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Pistachio ( Pistacia vera L.) Gum: a potent inhibitor of reactive oxygen species

机译:开心果(Pistacia vera L.)口香糖:一种有效的活性氧抑制剂

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In the present study, in order to evaluate antioxidant and radical scavenging properties of Pistachio gum (P-Gum), different bioanalytical methods such as DPPH? scavenging activity, DMPD?+ radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, reducing ability Fe3+–Fe2+ transformation, Cuprac and FRAP assays, scavenging by riboflavin-methionine-illuminate system and ferrous ions (Fe2+) chelating activities by 2,2′-bipyridyl reagent were performed separately. P-Gum inhibited 54.2% linoleic acid peroxidation at 10?μg/ml concentration. On the other hand, BHA, BHT, α-tocopherol and trolox, pure antioxidant compounds, indicated inhibition of 80.3%, 73.5%, 36.2% and 72.0% on peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, all of sample had an effective DPPH?, DMPD?+ and scavenging, Fe3+ reducing power by Fe3+–Fe2+ transformation and FRAP assay, Cu2+ reducing ability by Cuprac method and Fe2+ chelating activities.
机译:在本研究中,为了评估开心果胶(P-Gum)的抗氧化剂和自由基清除性能,采用了多种生物分析方法,如DPPH ?清除活性,DMPD ?+ 自由基清除活性,硫氰酸铁测定总抗氧化剂活性,还原能力Fe 3 + –Fe 2 + 转化,Cuprac和FRAP测定,核黄素-蛋氨酸-照明系统的清除分别进行了2,2'-联吡啶试剂对亚铁离子(Fe 2 + )的螯合活性的研究。在10?μg/ ml的浓度下,P-Gum可抑制54.2%的亚油酸过氧化。另一方面,纯抗氧化剂化合物BHA,BHT,α-生育酚和trolox分别表明在相同浓度下对亚油酸乳液的过氧化抑制分别为80.3%,73.5%,36.2%和72.0%。另外,所有样品均具有有效的DPPH ?,DMPD ?+ 和清除Fe 3 + 的Fe 3还原能力。 + –Fe 2 + 转化和FRAP分析,Cucuprac法还原Cu 2 + 的能力和Fe 2 + 的螯合活性。

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