Antimicrobial activities of Chaetomorpha antennina dichloromethane extract against isolated urinary tract infection pathogens

摘要

During the treatment of infectious diseases, upon repeated administration of an antibiotic, most microbial pathogens generate resistance to that antibiotic. Multiple drug resistance is also a widespread problem. Thus, it is the need of the hour to identify alternative antibacterial drugs. Seaweeds are one of the major repositories of numerous pharmacologically active secondary metabolites. The goal of this investigation is to evaluate the antimicrobial activity of Chaetomorpha antennina dichloromethane (DCM) extract against isolated urinary tract infection pathogens from urinary tract infection (UTI) infected patients. Antimicrobial activity, minimum inhibitory concentrations (MIC), minimum bactericidal or candicidal concentration (MBC/MCC) and biofilm inhibition activity of C. antennina extract was observed against the 7 isolated microbial strains: Enterococcus avium, Enterobacter cloacae, Staphylococcus hominis, Staphylococcus epidermidis, Pseudomonas aeruginosa, Proteus mirabilis, and Escherichia coli and Candida albicans (yeast). All isolated strains from UTI infected patients were later sequenced, identified and submitted in PubMed. The crude DCM extract of C . antennina showed remarkable inhibitory effects ranging between 2mm and 9mm, and its activity was recorded in the following order of E. coli E. avium E. cloacae and S . hominis S. epidermis P. aeruginosa P. mirabilis C. albicans. The MIC ranged between 500 to 750μg/ml, and the MBC was recorded in the range of 750 to 1000μg/ml. The crude DCM extract of C. antennina was also observed to have biofilm inhibitory activity at different concentrations against isolated UTI pathogens. This investigation elucidates that C. antennina DCM extract possesses antimicrobial activity against isolated UTI pathogens.