Antimicrobial resistance and selected beta-lactam resistance genes in Escherichia coli from canine urinary tract infections.

摘要

Little is known about antimicrobial resistance in bacteria from pet dog populations in Canada. Three hundred and sixty-three E. coli isolates were collected from cases of canine urinary tract infection in Ontario and Quebec, and their susceptibility to 15 antimicrobial agents was assessed. Resistance to ampicillin was the most frequent; no isolates were resistant to amikacin, The overall frequency of antimicrobial resistance was relatively high, 34% overall; there was no apparent difference between Ontario and Quebec. Multiresistance was common (17%), as was beta-lactam resistance (20%). blaTEM, blaCMY-2 , blaCTX-M and bla OXA-1 but not blaSHV genes were detected in isolates resistant to beta-lactams. Further characterization of blaCTX-M revealed two blaCTX-M-14 and blaCTX-M-15 variants. Isolates from two different canine patients belonged to a blaCTX-M-15 -carrying O25:H4 clone associated worldwide with human infections.