FORMULATION AND EVALUATION OF TROPICAMIDE IN-SITU GELS LOADED SOLID LIPID NANOPARTICLES FOR OCULAR DRUG DELIVERY

摘要

The aim of present work Formulation and Evaluation of Tropicamide In-situ Gels loaded Solid Lipid Nanoparticles for Ocular Drug Delivery. The surface morphological of SLN was carried out by TEM. The Tropicamide loaded solid lipid nanoparticles was measured the average particle size was ranges from 182.1+3.12nm to 390.1?±2.10 nm. The zeta potential ranges from -0.17?±1.4 mV to -3.80?±1.5 mV. The entrapment efficiency 66.2 % to 89.2 %. Drug content was ranges from 0.112mg/ml to 0.502 mg/ml. The percentage yield ranges from was ranges from 0.112mg/ml to 0.502 mg/ml. The polydispersity index ranged from 1.011?±0.15 to 1.327?±0.13. These SLN enriched in Chitosan gels the pH of the formulations range from 6.8 to 6.9. The gelling strength ranged from 129 sec to 152 sec. The bioadhesive force was ranges from 10.21 ?±1.15 dynes/cm2 to 15.23 ?± 1.22 dynes/cm2. The viscosity was ranges from 2212 ?± 1.14 cps to 2420?± 1.19 cps. The spreadability coefficient was ranges from 11.2 ?± 1.10 gms/sec to 13.3 ?± 1.21 gms/sec. The in-vitro diffusion release studies carried out at 12 hrs TSLNGF19 shows the 79.2 ?± 0.32. The ex vivo permeation studies for optimized formulation the increased drug permeation and corneal accumulation. In vitro corneal permeation profile of tropicamide loaded SLN from the chitosan gels and commercial eye drop solution (Tropicacyl) across the isolated porcine cornea. The ocular tolerance studies performed with HETCAM assay, corneal hydration study, histopathological studies. The stability studies of chitosan gels for long-term stability as per ICH guidelines (25?°C ?± 2?°C / 60% RH ?± 5% RH) &accelerated stability as per ICH guidelines (40?°C ?± 2?°C / 75% RH ?± 5% RH) there is no changes in gelling strength, bioadhesive force, viscosity, spreadability coefficient in optimized formulation.