Background/purpose The use of dental adhesives in everyday dental practice has raised questions about their biologic safety. Their biocompatibility is a relevant aspect of the clinical success of these materials. The objective of this study was to evaluate the genotoxicity of dental adhesives ex?vivo using a cytogenetic assay. Materials and methods Four materials (AdheSE, G-Bond, Excite, and Adper Single Bond 2) were tested on human peripheral blood leukocytes using a comet assay. Prepared materials were eluted in a saline solution for 1 hour, 1 day, and 5 days. The comet assay was used to evaluate primary DNA damage by measuring the tail length and tail intensity. A Kruskall-Wallis nonparametric test was used for the statistical analysis, with the significance level set to P?
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机译：背景/目的在日常牙科实践中使用牙科粘合剂已经引起了对其生物安全性的质疑。它们的生物相容性是这些材料临床成功的一个相关方面。这项研究的目的是使用细胞遗传学方法评估离体牙胶的遗传毒性。材料和方法使用彗星测定法在人外周血白细胞上测试了四种材料（AdheSE，G-Bond，Excite和Adper Single Bond 2）。将制备的物质在盐溶液中洗脱1小时，1天和5天。彗星试验用于通过测量尾巴长度和尾巴强度来评估初级DNA损伤。统计分析使用Kruskall-Wallis非参数检验，显着性水平设置为P <0.05。结果测试的牙科用胶粘剂均未显示经处理的白细胞的尾巴长度或尾巴强度有统计学显着增加，与所施加的稀释液，洗脱时间和聚合形式无关。对于所有测试的胶粘剂，在最低稀释度（1:10 2）下，洗脱期为1天和5天后，观察到DNA分子的尾巴长度和强度略有增加，只是处于非聚合形式。但是，这些结果在统计上并不显着。结论在本研究中使用的条件下，就遗传毒性而言，所有粘合剂均具有可接受的生物相容性。
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