Flow-Injection Chemiluminescence Determination of Fleroxacin in Pharmaceutical Preparations and Human Urine

摘要

Quality control of drug fleroxacin dosage, its monitoring in biological fluids, and research of drug’s metabolism and action are an important analytical task. A new chemiluminescence (CL) reaction system was established for the determination of fleroxacin (FLX). The trivalence dysprosium-sensitized CL emission mechanism was investigated by comparing the fluorescence emission with CL spectra. The CL spectra of FLX-KMnO 4 -Na 2 S 2 O 3 -H 6 P 4 O 13 system are from the narrow characteristic emission of Dy 3+ at 482 and 578 nm ( 4 F 9 → 6 H 15/2 , 4 F 9 → 6 H 13/2 ) through the energy transfer from the excited SO 2 * to analyte, followed by intramolecular energy transfer from analyte* to Dy 3+ . The optimum conditions for CL emission were investigated and optimized. The relationships between the relative CL intensity and the concentration of the studied analyte have good linearity. The detection limit for FLX was 3.0×10 -10 g/mL. The relative standard deviation is 2.0% for 11 determinations of FLX at 2.0×10 -6 g/mL. The proposed CL system has been successfully applied for the determination of FLX in the injections and urine sample with satisfactory result. .