Distribution of SPV genes, plasmid profiles and pulsotypes of Salmonella enteritidis isolates of animal and human origins in selected locations of Zimbabwe

摘要

A total of 49?Salmonella enteritidis?isolates identified by culture and biochemical tests were confirmed using multiplex polymerase chain reaction (PCR). The isolates were grouped into two, those with?Salmonella?plasmid virulence (spv)?gene and those without the?spv?gene. Plasmid and pulsed field gel electrophoresis (PFGE) analysis were performed on both groups. Of the 49?S. enteritidis?isolates identified, 39 possessed a?spvgene. Moreover, of the 39 isolates possessing the?spv?genes, 32 were from animals and 7 from humans. Those isolates without the?spv?genes comprised of 5 isolates from humans and 5 from animals. Plasmid analysis discriminated the isolates with the?spvgenes into 11 profiles and isolates without?spv?gene into 3 profiles. PFGE discriminatedS. enteritidis?isolates into 10 pulsotypes (9 from animals and 1 from the human outbreak strains). There was no correlation between the pulsotypes or plasmid profiles with the geographical location of isolate. However, there was a link between strain type and source of isolate. The study was performed to characterize?S. enteritidis?strains using molecular typing techniques and determine their geographical distribution in Zimbabwe. The information obtained can give background information for further studies onSalmonella?epidemiology in Zimbabwe.