Cell Counting: How Reliable (Are These Methods?)

摘要

Cell counting is an inevitable step involved in experiments of many cell–based research studies.? The technique allows standardization of cell concentration between samples with minimized error and varia-tion in downstream results. Manual cell counting technique has been the most popular in use. Recently, technological improvement in cell imaging has lead to the manufacture of automated cell counters. The advancements in imaging technology, enabled by automated cell counters such as Countess? and LUNA-FLTM, are improving experimen-tal accuracy and reliability, with much less time and effort. This review explores different types of cell counting methods with more emphasis on the most recent counting method—the automated cell counters. The methodology, accuracy, precision, benefits and drawbacks of these methods are also highlighted.