Prospects for Creating Bioartificial Liver System with Induced Pluripotent Stem Cell Technology

摘要

The apple, Malus domestica Borkhausen has therapeutic potential by its action as free radical scavengers preventing oxidation of chemicals and their harmful effects on cells. Liposomes use as controlled releasing system of active ingredients is a new possibility. Santa Catarina produces 59% of Brazilian apples,therefore a study for an alternative use of the skin as a by-product from apple processing has been carried out. The work involved the extraction and quantification (Folin-Ciocalteau method) of total phenolics from Fuji apple skin with or without preservative addition; preparation of liposome with apple skin extract. Galenic bases were tested (Polavax", Lanete and Gel cream) with the incorporation of the liposome with extract without preservative (A) and with preservative (B), in subsequent application primary stability test. Extraction performed with preservative had higher concentration of phenolics, suggesting the delay of oxidation process. However, should rule out the possibility of any interference of the preservatives in the quantification method used. It was well succeeded in the preparation and incorporation of liposome with phenolics from apple skin on the galenic bases, Polavax" showed the best performance. By-product “apple skin” proved to be a good source of phenolics, tests of chemical contaminants must be conducted in the future studies.