Growth and expression level of growth hormone in transgenic mutiara catfish second generation

摘要

Growth improvement through a selection program on African catfish has been conducted in Indonesia but the genetic gain achieved was still low. This study was aimed to evaluate the growth and the expression level of African catfish growth hormone (CgGH) gene in transgenic Mutiara catfish second generation (G2). Three G1 transgenic Mutiara broodstock were selected based on CgGH expression level and spawned to produce G2. A pair of non-transgenic was provided as control. The identification of G2 transgenic fish was performed using the PCR method, while the analysis of CgGH expression level was done by using the semi-quantitative RT-PCR method. The results showed that one of the three G1 transgenic fish transmitted the CgGH transgene following the Mendelian segregation pattern. The level of CgGH expression in caudal fin, muscle, and liver tissues of G2 fish varies among crossing and individuals from the same G1 broodstock. The growth of G2 transgenic Mutiara catfish was 3-4 times higher than non-transgenic fish (p ? 0.05). The feed conversion ratio of G2 transgenic fish were 50.3-64.5% lower than that of non-transgenic fish. Thus, this CgGH transgenic Mutiara catfish is most likely potential to be cultured to increase productivity and efficiency of cultivation.