Anti-Herpes Simplex Virus Effect of Camellia sinesis, Echiumamoenum and Nerium oleander

摘要

As viral resistance to available chemical drugs and virus latency duration causes problems in the treatment of herpes simplex virus type 1 infections, there is an evolving need for new anti-Herpes drugs. The present study evaluated the antiviral effect of Camellia sinesis, EchiumamoenumL and Nerium oleander with ethno medical background on HSV-1 multiplication. Plants were extracted with decoction method to obtain aqueous extracts. These extracts were screened for their cytotoxicity against Hep-2 (Human epithelial type 2) cell line by cytopathic effect (CPE) assay. Antiviral effects of the plant extracts were determined by Neutralization Test (NT) at times one, two and three hour. Nerium oleander extract had most toxicity (> 5 μg/ml) on cell line, and Camelliasinesis showed the most anti-Herpes property at inhibition of HSV-1 multiplication at one and two hour that decreased at three hour. EchiumamoenumL had lowest anti-Herpes effect that at two hour was similar antiviral property of Camelliasinesis at three hour. Camellia sinesis and EchiumamoenumL showed the most anti-Herpes effect when they used an hour after virus inoculation. Further research is needed to elucidate the active constituents of these plants which may be useful in the development of new anti-Herpes drugs.