The objective of the present study was to investigate the effect of pre-milking mechanical teat stimulation on milk yield and milking performance of dairy buffaloes in early lactation. For this purpose, twenty-two healthy Italian Mediterranean buffaloes in their first to third lactation and in early lactation (<120 days in milk) were subjected to two treatments of teat stimulation: i) washing of the teats with water for about 5 s and attaching of the milking unit within 60 s, without any pre-milking massage (farm milking routine); ii) fast pulsation (FP), achieved by increasing pulsation rate to 120 pulsations per min during the first 60 s after application of teat cups. Each treatment lasted for 10 days and the following parameters were measured: milk yield (kg/milking), milk yield at 2 min after unit attachment (kg), time between milking unit attachment and its automatic removal (min), peak milk flow rate (kg/min), and milking time to reach peak flow rate (min). The average milk flow rate (kg/min) was calculated by dividing milk yield by the actual milking time. Milk yield was not affected by mechanical pre-stimulation and during the first 2 min of milking 20.2% and 19.6% of total milk yield were milked respectively when treatments 1 and 2 were applied. The time elapsed from attachment of the milking cluster until its automatic removal was less than 8 min both for buffaloes subjected to FP, and for buffaloes subjected just to washing of the teats before attaching the milking unit. FP stimulation did not show an enhancing effect on peak and average milk flow rates and on milking time to reach peak flow rate.
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