Molecular Cloning, Structural Analysis and Tissue-Specific Expression of Akirin1 Gene in Tianfu Goat

摘要

Akirin1 gene is a newly discovered nuclear factor which plays an important role in myogenesis and closely contact with meat production. For better understanding of the structure and functions of the Akirin1 gene and to study its effect in Capra hircus, the cDNA of Tianfu goat Akirin1 gene was cloned and sequenced. The structure of Akirin1 was analyzed using bioinformatics tools. The results showed that the Tianfu goat Akirin1 cDNA full Coding Sequence (CDS) contained 426 bp nucleotides and encoded 141 amino acids protein. Phylogenic tree analysis revealed the goat Akirin1 closely related with cattle Akirin1. Quantitative real-time PCR (qPCR) analyses showed that Akirin1 was expressed in the myocardium, liver, spleen, lung, kidney, leg muscle, abdominal muscle and longissimus dorsi muscle. Especially, high expression levels of Akirin1 were detected in liver, spleen, lung and kidney and low expression levels were seen in myocardium, leg muscle, abdominal muscle and longissimus dorsi muscle. Western blotting results showed Akirin1 protein expression only detected in lung and three skeletal muscle tissues.