A Novel Pig Gene, PFKFB1, Differentially Expressed in the Muscle Tissues from Wujin Pigs and Large White Pigs

摘要

The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large white pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the Rapid Amplification of Cdna Ends (RACE) method. The open reading frame of this gene encodes a protein of 428 amino acids which is homologous with the 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 1 (PFKFB1) of 6 species: cattle (97%), human (97%), horse (96%), rhesus monkey (96%), rat (94%) and mouse (94%). This newly identified gene was respectively defined as the swine PFKFB1 gene and had been assigned GeneID: 100233197. The phylogenetic tree analysis revealed that the swine PFKFB1 gene has a closer genetic relationship with the PFKFB1 gene of cattle. The tissue expression analysis indicated that the swine PFKFB1 gene has a broad tissue distribution. The experiment is the first to establish the primary foundation for further research on the swine PFKFB1 gene.