In vitro propagation of Gymnema sylvestre Retz. R.Br through apical bud culture

摘要

Gymnema sylvestreRetz. R.Br. is a very important medicinal plant used in many medical formulations mainly for the treatment of diabetes. The extensive use ofG. sylvestrehas resulted in its over-exploitation and the plant is rare in many states of India. In the present study multiple shoot were regenerated using apical buds as an explant. Several concentrations of cytokinins viz. kinetin and BAP were attempted with MS medium for efficient shoot induction. Highest shoot frequency was obtained in MS medium fortified with BAP (4.44 µM) and KN (4.64 µM) with 3% sucrose. Shoots obtained were rooted using half strength MS media with IAA. Eighty five percent of rooted shoots survived in the field.