Viscosimetric study and synergistic antifungal activity of oils extract from three Algerian medicinal plants against toxigenic Aspergillus

摘要

Oils extractedfrom seedsof threemedicinal plantsused in Algerian folk medicine(Citrullus colocynthis,Nigella sativaandLinumusitassimum),weretested for theirantifungalsynergyagainstonestrain ofAspergillusflavustoxin(A. flavusMTTC2799)by the method ofradial growthon solid mediumPotateosDextrose Agar(PDA).Antifungal results revealedthatall oilstestedatthe concentration1/5000inhibited thegrowthof the studied fungus, where the bestantifungalpotency was observed for theL.usitassimumseed oil.The values ofantifungalindices(55.65, 48.65,40.54)wererecorded,respectivelyforL. usitassimumoil,C. colocynthisoilandN. sativaoil at concentrations of (1/5000, 1/500 and 1/2000).The resultsof the synergistic antifungal activityobtained byoils combinationrevealed thatL.oil/C.oilshowedthe bestantifungal effect (54.19%) before theL.oil/C.oil/N.oil system with 48.76% of inhibition. In order to explain the synergistic effect and the best behavior of oils, especially determination of optimal concentrations C* (C star) needed for antifungal synergisticactivity,viscosimetric investigation was carried out at room temperature and at several concentrations.