Bioactivity of natural compounds isolated from cyanobacteria and green algae against human pathogenic bacteria and yeast

摘要

Analysis of bioactive natural compounds of algae such as cyanobacteria (Spirulina platensis;Nostoc linckia;Phormidium autumnale;Tolypothrix distortaandMicrocystis aeruginosa) and green algae (Chlorella vulgarisandDunaliella salina), and their activity against human pathogenic bacteria and yeast such asSalmonella suisATCC 13076;Pseudomonas aeruginosaATCC 27583;Escherichia coliATCC 25922;Staphyllococcus aureusATCC 25923;Bacillus subtilisATCC 6633;Shigella sonneiATCC 11060 andCandida albicansATCC 10231 have been studied in Saudi Arabia. Extraction of algal metabolites was performed by using the mixture of three organic reagents, methanol:acetone:diethyl ether as 5:2:1 v/v, respectively. All metabolites of algae isolates had shown weak to strong antimicrobial activity toward one or more human pathogenic microorganisms. Almost all the algal extract showed strong activity againstS. sonneiin agar well diffusion technique. Crude extract of cyanobacteria,T. distortashowed moderate to strong activity againstS. aureus, B. subtilisandS. sonnei. Further, crude extract of all the algal metabolites have been analyzed using gas chromatography-mass spectrometry (GC-MS). Results indicated that the main component in the crude extracts ofP. autumnaleis 1-Hexyl-2-Nitrocyclohexane (91.7%);C. vulgarisis 2-Butanol, 3-methyl-, (S)- (90.8%);S. platensisis Nitrocyclohexane-2-Hexyl-1 (92.1%);N. nostocis Octadecanal (aldehyde) (86.8%);D. salinais 3-Methyl-2-(2-Oxopropyl) Furan (90.%);T. distortais Boronic acid, Ethyl-, Dimethyl ester (83.9%) and lastlyM. aeruginosais (S)-(+)-1-Cyclohexylethylamine representing 91.9%, respectively. Further, in this study, the extracts of all the algal species especiallyT. distorta,P. automonate,C. vulgarisandD. salinahave been found potential for the production of several compounds including biomedically important organic metabolites such as ethane,1,1-diethoxy-; butanal; heptanal and octanal. Further study for the purification of the potent compound will explain their usefulness in the pharmaceutical and biotechnological industry.