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Astragaloside IV protects rat retinal capillary endothelial cells against high glucose-induced oxidative injury

机译:黄芪甲苷IV保护大鼠视网膜毛细血管内皮细胞免受高糖诱导的氧化损伤

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Aim: Diabetic retinopathy is a microvascular complication of diabetes that leads to blindness. Hyperglycemia causes oxidative stress, which is an important cause in the pathogenesis of microangiopathy. The aim of this study was to investigate the potential protective effects of astragaloside IV (AS-IV) in retinal capillary endothelial cells (RCECs) incubated with high glucose conditions. Methods and results: Based on rat RCECs cultured with high glucose (30 mM) in vitro, a significant increase in cell viability in rat RCECs incubated with both AS-IV and high glucose for 48 or 72 h by MTT assay. The increased viability was accompanied by decreased glucose transporter-1 expression using immunofluorescent assay. Meanwhile, AS-IV reduced intracellular hydrogen peroxide and superoxide, decreased mitochondrial reactive oxygen species in rat RCECs with high glucose by the fluorescent probes, and lowered malondialdehyde levels. In addition, AS-IV increased the activities of total superoxide dismutase, MnSOD, catalase, and glutathione peroxidase. The glutathione content also increased after AS-IV treatment. Furthermore, AS-IV reduced NADPH oxidase 4 expression by western blot method. Conclusion: These results suggest that the main mechanism underlying the protective effects of AS-IV in high glucose-injured RCECs may be related to its antioxidative function.
机译:目的:糖尿病性视网膜病是糖尿病的微血管并发症,导致失明。高血糖症会引起氧化应激,这是微血管病发病机理中的重要原因。这项研究的目的是研究黄芪甲苷IV(AS-IV)对在高葡萄糖条件下培养的视网膜毛细血管内皮细胞(RCEC)的潜在保护作用。方法和结果:基于体外培养的高浓度葡萄糖(30 mM)的大鼠RCEC,MTT分析显示,与AS-IV和高葡萄糖同时培养48或72 h的大鼠RCEC细胞活力显着增加。使用免疫荧光测定法,增加的生存力伴随着葡萄糖转运蛋白-1表达的降低。同时,通过荧光探针,AS-IV可以降低高糖大鼠RCEC中的细胞内过氧化氢和超氧化物,降低线粒体活性氧,并降低丙二醛水平。此外,AS-IV增加了总超氧化物歧化酶,MnSOD,过氧化氢酶和谷胱甘肽过氧化物酶的活性。 AS-IV处理后,谷胱甘肽含量也增加。此外,AS-IV通过蛋白质印迹法降低了NADPH氧化酶4的表达。结论:这些结果表明,AS-IV对高葡萄糖损伤的RCEC保护作用的主要机制可能与其抗氧化功能有关。

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