Reverse phase high-performance liquid chromatographic method development for simultaneous determination of biologically active and thermally stable 1,1a??-bis(3-methyl-4-hydroxyphenyl)cyclohexane and 1,1a??-bis(3,5-dibromo-4-hydrophenyl)cyclohexane

摘要

A rapid and sensitive reverse phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated successfully for the simultaneous determination of biologically active and thermally stable 1,1’-bis(3-methyl-4-hydroxyphenyl) cyclohexane (MEBC) and 1,1’-bis(3,5-dibromo-4-hydrophenyl)cyclohexane (TBBC). Separation of analytes were achieved within 10min by using Phenomenex Luna C18(2) (25mm x 4.26mm, 5 μm) column. The method has excellent linearity (R2 ?? 0.9990), precise (RSD ?? 2), accurate (recovery of 99.43-99.76 % for MEBC and 99.69-100.12 % for TBBC, specific and robust. Limit of detection and limit of quantitation were 1.5 μg mL-1and 4.0μg mL-1, respectively. The developed method may be useful bioavailability and bioequivalence studies.