Quantitative estimation of gallic acid and tan

摘要

Bhuvnesvara vati (BV) is an Ayurvedic formulation containing Emblica officinalis, Terminalia belerica, Terminalia chebula, Trichyspermum ammi, Aegle marmelus and two minerals namely rock salt and Soot as main ingredients. The study was aimed to develop finger printing methods for well-known Ayurvedic formulation. Three sample batch of BV were prepared in the laboratory and two different marketed formulations were procured from Ayurvedic medicine shop. A HPLC method was developed for the estimation of gallic acid and tannic acid in laboratory and marketed formulations. The concentration of gallic acid present in raw material is found to be 3.174±0.049% w/w in Emblica officinalis, 8.920±0.173% w/w in Terminalia belerica, 4.092±0.117% w/w in Terminalia chebula, 1.831±0.973% w/w in Aegle marmelus and 0.264±0.365% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356% and 2.632±0.239% w/w respectively. Two marketed formulation of Bhuvnesvara vati M-I and M-II showed gallic acid concentration to be 2.019±0.872 % and 2.019±0.872 % w/w respectively. The concentration of tannic acid present in raw material was found to be 6.172%±0.365w/w in Emblica officinalis, 8.667%±0.0319w/w in Terminalia belerica, 13.956%±0.745w/w in Terminalia chebula, 4.789±0.983% w/w in Aegle marmelus and 0.668±1.002% w/w in Trichyspermum ammi respectively and in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356%, 2.632±0.239% w/w respectively. In order to obtain precision and accuracy, the recovery study was performed and result obtained with mean value 99.69% for gallic acid and 99.38% for tannic acid, which prove reproducibility of the result. This show significant precision of methods at 95% confidence level. The mean of % RSD value was found to be 0.357 for gallic acid & 0.353 for tannic acid. Results of statistical analysis shows that present HPLC method for determination of gallic acid and tannic acid is simple, precise, accurate and suitable for routine analysis of gallic acid and tannic acid in BV. The developed fingerprints can be used as a standard and gallic acid and tannic acid can be used as a possible marker compound for fingerprinting of BV.