Phytochemical analysis, in-vitro screening for antimicrobial and anthelminticactivity of combined hydroalcoholic seed extracts of four selected folkloreindian medicinal plants

摘要

Antimicrobial properties of medicinal plants are being increasingly reported from different parts of the world.The main aim of present research work is to investigate Invitro antimicrobial activity, anthelmintic activity and Preliminary screening for phytochemical constituents of the hydroalcoholic extract of Coriandrum sativum, Cassia occidentalis, Carica papaya,Moringa foetida). Anti- microbial assay was evaluated using Nutrient agar medium and Disc diffusion method at different concentrations (50,100,150,500mg/ml) and the results were compared with therapeutically used antibiotics. Rapid formation of Inhibition zones within 24 hours of incubation was obtained with Hydroalcoholic extract 50mg/ml. Anthelmintic activity was evaluated using adult Indian earthworms, Pheretima posthuma and Tubifex tubifex, having anatomical and physiological resemblance with intestinal round worms parasite. The earthworms were washed in normal saline solution before they were released in to 10ml of respective formulation as follows, vehicle (2%v/v Tween 80 in normal saline) and piperazine citrate (10mg/ml)and prototypes(10,20,50mg/ml). All the Investigations on Hydroalcoholic extract exhibited Antimicrobial and Anthelmintic activity at 20mg/ml with significant activity (P0.05). The MIC of Hydroalcoholic was determined using the parameters like time of paralysis and time of death with the extract at concentrations of 10,20,50mg/ml.The extract exhibited significant anthelmintic activity at highest concentrations of 50mg/ml as compared with piperazine citrate (10mg/ml) as standard reference and distilled water as control.Herbal drugs and synthetic drugs were equally effective in helminthic infestations but Hydroalcoholic extract of Indian medicinal plants exhibits potentiality and have maximum anthelmintic activity.