Simple, accurate, rapid and highly sensitive Extractive Spectrophotometeric determination method has been developed and validated for estimation of Amphetamine Sulphate in pharmaceutical solid dosage form. The proposed method is based on the principle that Mono and diamines in the dissociated form (Ion) combined with dissociated form of the Acid dye Solochrome black T. The formation of Acid dye complex has been evaluated by carrying TLC, Infra-red spectra. The acid dye-drug complex has lmax at 520 nm. Beer’s Lambert Law obeyed in the range of 20-100μg ml-1. The results were validated statistically. The further scope for this method is that it can be applied to body fluid in cases of intoxication.
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机译:已经开发了简单,准确,快速和高度灵敏的萃取分光光度法测定方法,并已用于估算药物固体剂型中的苯丙胺硫酸盐含量。所提出的方法基于这样的原理,即解离形式(离子)的单胺和二胺与酸性染料Solochrome black T的解离形式结合。已通过携带TLC,红外光谱评估了酸性染料配合物的形成。酸性染料-药物复合物在520nm处具有1max。啤酒的朗伯定律遵循20-100μgml-1的范围。结果经过统计学验证。该方法的进一步范围是在中毒的情况下可以应用于体液。
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