synergic application effects of silver nanoparticles and low-frequency electromagnetic field on the chick embryo chorioallantoic membrane on angiogenesis

摘要

Background and purpose:  Angiogenesis has a role in many vital processes also many developmental processes are influenced by an electromagnetic field. In the present study the synergic effects of silver nanoparticles and low-frequency electromagnetic field on angiogenesis in the chick embryo chorioallantoic membrane (CAM).Materials and method: In this experimental study, (Ross strain ) on the eighth day incubation of embryonated chicken eggs were treated with 50, 100 and 200 mg/ml of silver nanoparticles. Then on tenth day, the eggs for 3 hours were exposed to the electromagnetic field. The twelfth day, the samples were photographed and the number, length of vessels, weight and Crown-rump were analyzed with software Image J. hemoglobin content were tested with derabkin assay. Silver nanoparticles antioxidant activity was assessment. Data were analyzed using spss 16 software and ANOVA statistical test.Results: The average number and length of vessels and hemoglobin content decreased in treated groups with silver nanoparticles and Electromagnetic fields. synergic use of silver nanoparticles and Electromagnetic fields reduce the average number and length of vessels and hemoglobin content  than using one alone. Antioxidant assay showed that silver nanoparticles inhibited free radical production.Conclusions: Synergic application of biogenic silver nanoparticles with electromagnetic field significantly reduced angiogenesis CAM comparing to use one of them.