AN EFFICIENT, SCALABLE PATIENT-DERIVED XENOGRAFT SYSTEM BASED ON A CHICK CHORIOALLANTOIC MEMBRANE (CAM) IN VIVO MODEL

摘要

Embodiments of the disclosure encompass systems that utilize chick chorioallantoic membranes (CAM) as models for cancer xenografts, including at least patient-derived xenografts (CAM-PDX). In particular embodiments, the system employs the CAM-PDX to graft and culture different types of tumor tissue on a single or multiple eggs. In specific embodiments multiple tumor regions of a single tumor are cultured on a single egg. Frozen tissue is successfully revived, in specific embodiments. Downstream applications following successful establishment of CAM-PDX models are encompassed.