Examination and Comparison of Various Methods of DNA Extraction from Samples of Blood, Bone Marrow Slides and Peripheral Blood

摘要

Introduction: DNA extraction methods are very important for genetics research and diagnostic tests. In These methods are also important for detecting genetic diseases or in cancers for investigating genetic changes during cancer progression or treatment. Therefore, selection of the best method for DNA extraction from different samples such as bone marrow (BM) or peripheral blood (PB) and their slides is very important. Methods: In the present research, DNA was extracted from 5 different samples including 1-PB, 2-BM slides stained by Gimsa method, 3-Gimsa stained PB slides from archives, 4-new Gimsa stained PB slides and 5-non stained new PB slides by 3 different methods salting-out, boiling and phonal chloroform method. In all of the groups, three DNA parameters were investigated 1-OD (Optimal Density), 2-DNA concentration and 3-Outcome (PCR results).Results: The best DNA quality was achieved by salting-out method (OD=1.74), while the worst quality was by the boiling method (OD