Lysosomal enzymes in rat sarcomas induced by 7,12‐dimethylbenz(α)anthracene and Rous sarcoma virus

摘要

The lysosomal apparatus in normal rat fibroblast cultures and in DMBA and RSV induced rat sarcoma cell cultures was compared. Both DMBA and RSV induced sarcoma cells showed relative increase of lysosomal enzyme activity outside the cells in the medium compared to the activity within the cells. An early equilibrium of enzyme activity level was reached in the medium from RSV sarcoma cells, whereas this was not seen in cultures of normal fibroblasts and DMBA sarcoma cells. There was a difference in the intracellular content of lysosomal enzymes between RSV and DMBA sarcoma cells. This was most clearly seen for N-acetyl-β-glucosaminidase. These findings and the possible role of lysosomal enzymes in malignant cells are discussed.