7,12-Dimethylbenz(a)anthracene-Deoxyribonucleoside Adduct Formation 'in vivo': Evidence for the Formation and Binding of a Monohydroxymethyl-DMBA (Dimethylbenz(a)anthracene) Metabolite to Rat Liver DNA (Deoxyribonucleic Acid)

摘要

The polycyclic aromatic hydrocarbon, 7,12-dimethyl benz(a)anthracene (DMBA) is a potent carcinogen to the female Sprague-Dawley rat, and when administered under conditions that have been shown to produce cancer, results in extensive formation of hydrocarbon-deoxyribonucleoside adducts. Sephadex LH-20 and reverse-phase h.p.l.c. and spectrofluorometric analysis of these adducts demonstrate that at least one adduct results from the binding of 7,12-dimethylbenz(a)anthracene-1,2,3,4-tetrahydro-3,4-dihydroxy-1,2- oxide.