RAPD Analysis of Genetic Variation in Eucalyptus Microtheca F. Muell. Populations

摘要

Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic variability in Eucalyptus microtheca F. Muell. (family Myrtaceae), a native Australian species grown in the arid and semi-arid zones. Twelve natural populations from widely-separated locations were analyzed with 18 primers. The primers yielded a total of 102 polymorphic bands, i.e. an average of 6 polymorphic bands per primer. Molecular markers were used to calculate the similarity coefficients, which were then used for determining genetic distances between the populations. Based on genetic distances, a dendrogram was constructed. Gene diversity values for each population ranged between 0.176 (the population from southeastern Australia) and 0.232 (the population from western Australia) with an average of 0.200. Total gene diversity for this species was 0.240, where 83.3% of the variation was found within populations and 16.7% between populations.