A combination treatment approach and cord blood stem cell transplant for blastic plasmacytoid dendritic cell neoplasm | Haematologica

摘要

Key-wordsWe read with great interest the article on blastic plasma-cytoid dendritic cell neoplasm (BPDCN) by Pagano et al.1 The authors propose a strategy of combining ALL-like and AML-like protocols for this rare and aggressive disease. The authors also emphasize the importance of consolidating when possible with an allogeneic stem cell transplant, in light of the poor prognosis (median overall survival of 8.7 months) of BPDCN. We would like to share here our experience of successfully treating a patient with cord blood stem cell transplantation using a preparative regimen of thiotepa, fludarabine and melphalan.2Our patient is a 39-year old female who presented with a history of easy bruising and shortness of breath. No skin lesion or lymph node enlargement was noted at the time of diagnosis. Peripheral blood examination showed pancytopenia. Absolute neutrophil count was 0.9×109/L, hemoglobin 7.9g/dL, platelet count 21×109/L, and there was no evidence of blasts in the peripheral blood. A subsequent bone marrow aspirate and biopsy confirmed a diagnosis of BPDCN. The blasts in the bone marrow were positive for CD2, CD4, CD7, CD8, CD56, CD64, HLA-DR by flow cytometry analysis and positive for CD123 and TCL13 by immunoperoxidase staining. The blasts were negative for CD34, TdT, CD13, CD33, CD10, as well as for lineage specific markers including CD20, CD19, cytoplasmic CD22 for B cells, surface CD3, cytoplasmic-CD3 for T cells, and myeloperoxidase for myeloid cells. Fluorescence in situ hybridization for EBV encoded RNA (EBER) was also negative. Although CD8 expression is not typical, the diagnosis of BPDCN was favored over aggressive NK-cell leukemia based on agranular cytoplasm of blasts and lack of EBV expression. Cytogenetic analysis showed a normal female karyotype with no cytogenetic aberrations. Cerebrospinal fluid (CSF) cytology showed no evidence of involvement by the malignant cells.The patient was initially treated with a standard regimen for aggressive T/NK cell malignancies called “SMILE” that consisted of steroid (dexamethasone), methotrexate, ifosfamide, L-asparaginase and etoposide.4 Day 21 bone marrow biopsy after the first cycle showed a partial response (PR) based on a greater than 50% reduction in blast percentage (from 60% down to 20%). She was subsequently treated with an AML regimen that consisted of high-dose cytarabine and mitoxantrone.5 She achieved a complete remission (CR) and was consolidated 11 weeks after diagnosis with a cord blood stem cell transplant. The preparative regimen consisted of thiotepa (5 mg/kg) Day -7, fludarabine (30 mg/m2) Day -6 through Day -2, melphalan 140 mg/ m2 on Day -1 and rabbit ATG (3 mg/kg).6 A single cord blood unit with 3.81x107 total nucleated cells/kg and 2.95x105 CD34 cells/kg was infused on Day 0. The unit was 4 of 6 HLA matched with the patient based on HLA A, B and DR typing. Graft-versus-host disease (GVHD) prophylaxis consisted of tacrolimus and mycophenolate mofetil. The patient engrafted her neutrophils on Day 18 and platelets on Day 39 post transplant. One hundred percent donor chimerism was achieved by Day 100. She is currently at 313 days (10.4 months) since her diagnosis and 237 days (7.9 months) since her cord blood stem cell transplant. She is being followed as an outpatient on tapering doses of immunosuppression. She is doing well except for mild grade 1-2 skin GVHD that has been responsive to topical steroids.Due to the scarsity of data and lack of consensus as to the optimal treatment approach for BPDCN, we used an approach that combined ALL-like and AML-like treatment protocols in sequence and achieved a complete remission. Since our patient did not have a matched related or unrelated donor, she underwent a cord blood stem cell transplant. This is the first case report demonstrating the successful use of a cord blood stem cell donor in this rare and aggressive disease.A sequential combination approach alternating ALL-and AML-like induction regimens